Background: Endodontic therapy does treat the tooth from inside and its achievement is based on comprehensive canal debridement, effective disinfection, and complete obturation of the canal space. The present study was conducting to compare the efficiency of single file rotary & reciprocating systems in reducing E. faecalis colony count after instrumenting.Material and methods: The present study was conducted to compare the efficiency of single file rotary & reciprocating systems in reducing E.faecalis colony count after instrumenting. For study forty two freshly intact human mandibular premolar teeth (length 20-21 mm), straight, with radiographically confirmed single root canal and fully formed apices, were obtained. The teeth were stored in 10% formalin until use. Before the commencement of study ethical approval was taken from the Ethical Committee. The coronal access was performed 42 specimens were divided into 3 groups(n=14) based on the instrumentation technique used. Irrigation protocol was to use 2ml of 3% sodium hypochlorite and 5ml of distilled water, except group 3. Group 1 One Shape files with 6% taper, Group 2 WaveOne files with the same amount of taper associated, Group 3 canals were uninstrumented (Negative control group). Samples from each root canal were tested to verify the presence or absence of the microbial growth both before and after final disinfection procedures. Statistical analysis was done using Statistical Package for the Social Sciences, version 21 (SPSS, Chicago, IL). Results: In present study total sample size was 42, which were divided into three groups. Our study shows that group II shows greater efficiency in reducing bacterial load than group I and group III. Conclusion: Our study concluded that WaveOne files were better than One shape files in reducing bacterial load.
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